Embryonic Stem Cells Exhibit mRNA Isoform Specific Translational Regulation.

ABSTRACT: The presence of multiple variants for many mRNAs is a major contributor to protein diversity. The processing of these variants is tightly controlled in a cell-type specific manner and has a significant impact on gene expression control. Here we investigate the differential translation rates of individual mRNA variants in embryonic stem cells (ESCs) and in ESC derived Neural Precursor Cells (NPCs) using polysome profiling coupled to RNA sequencing. We show that there are a significant number of detectable mRNA variants in ESCs and NPCs and that many of them show variant specific translation rates. This is correlated with differences in the UTRs of the variants with the 5'UTR playing a predominant role. We suggest that mRNA variants that contain alternate UTRs are under different post-transc

SUBMITTER: Wong QW 

PROVIDER: S-EPMC4723142 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature