Longitudinal Survey of Microbiota in Hospitalized Preterm Very-Low-Birth-Weight Infants.

ABSTRACT:

Objectives

The aim of the present study was to examine the changes in bacteria in hospitalized preterm infants during the first month of life.

Methods

Rectal swabs were collected daily from 12 preterm infants. DNA was extracted from swabs from day of birth and weekly thereafter. Bacterial taxa were identified with next generation sequencing using universal bacterial primers targeted at the 16S ribosomal DNA on a 454 Roche titanium platform. Sequences were clustered into operational taxonomic units, and taxonomy was assigned against the Greengenes databank using Quantitative Insights Into Microbial Ecology version 1.4. Quantitative polymerase chain reaction was used to determine the abundance of Bifidobacterium spp. Functional assessment of the microbiome was performed with P

SUBMITTER: Patel AL 

PROVIDER: S-EPMC4724288 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature