Electroporation of Brain Endothelial Cells on Chip toward Permeabilizing the Blood-Brain Barrier.

ABSTRACT: The blood-brain barrier, mainly composed of brain microvascular endothelial cells, poses an obstacle to drug delivery to the brain. Controlled permeabilization of the constituent brain endothelial cells can result in overcoming this barrier and increasing transcellular transport across it. Electroporation is a biophysical phenomenon that has shown potential in permeabilizing and overcoming this barrier. In this study we developed a microengineered in vitro model to characterize the permeabilization of adhered brain endothelial cells to large molecules in response to applied pulsed electric fields. We found the distribution of affected cells by reversible and irreversible electroporation, and quantified the uptaken amount of naturally impermeable molecules into the cells as a result of appl

SUBMITTER: Bonakdar M 

PROVIDER: S-EPMC4724630 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature