Gene expression profiling of gastric cancer by microarray combined with laser capture microdissection.

ABSTRACT:

Aim

To examine the gene expression profile of gastric cancer (GC) by combination of laser capture microdissection (LCM) and microarray and to correlate the profiling with histological subtypes.

Methods

Using LCM, pure cancer cells were procured from 45 cancerous tissues. After procurement of about 5000 cells, total RNA was extracted and the quality of RNA was determined before further amplification and hybridization. One microgram of amplified RNA was converted to cDNA and hybridized to cDNA microarray.

Results

Among 45 cases, only 21 were qualified for their RNAs. A total of 62 arrays were performed. These included 42 arrays for cancer (21 cases with dye-swab duplication) and 20 arrays for non-tumorous cells (10 cases with dye-swab duplication) with universal referen

SUBMITTER: Wu MS 

PROVIDER: S-EPMC4725172 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature