Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function.

ABSTRACT: The repair of wounds through collective movement of epithelial cells is a fundamental process in multicellular organisms. In stratified epithelia such as the cornea and skin, healing occurs in three steps that include a latent, migratory, and reconstruction phases. Several simple and inexpensive assays have been developed to study the biology of cell migration in vitro. However, these assays are mostly based on monolayer systems that fail to reproduce the differentiation processes associated to multilayered systems. Here, we describe a straightforward in vitro wound assay to evaluate the healing and restoration of barrier function in stratified human corneal epithelial cells. In this assay, circular punch injuries lead to the collective migration of the epithelium as coherent sheets. The c

SUBMITTER: Gonzalez-Andrades M 

PROVIDER: S-EPMC4725353 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature