Isolation of highly enriched primary human microglia for functional studies.

ABSTRACT: Microglia, the resident macrophages of the central nervous system play vital roles in brain homeostasis through clearance of pathogenic material. Microglia are also implicated in neurological disorders through uncontrolled activation and inflammatory responses. To date, the vast majority of microglial studies have been performed using rodent models. Human microglia differ from rodent counterparts in several aspects including their response to pharmacological substances and their inflammatory secretions. Such differences highlight the need for studies on primary adult human brain microglia and methods to isolate them are therefore required. Our procedure generates microglial cultures of >95% purity from both biopsy and autopsy human brain tissue using a very simple media-based culture proce

SUBMITTER: Rustenhoven J 

PROVIDER: S-EPMC4725991 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature