Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging.

ABSTRACT: Cells are complex chemical systems, where the molecular composition at different cellular locations and specific intracellular chemical interactions determine the biological function. An in-situ nondestructive characterization of the complicated chemical processes (like e.g. apoptosis) is the goal of our study. Here, we present the results of simultaneous and three-dimensional imaging of double organelles (nucleus and membrane) in single HeLa cells by means of either labelled or label-free surface-enhanced Raman spectroscopy (SERS). This combination of imaging with and without labels is not possible when using fluorescence microscopy. The SERS technique is used for a stereoscopic description of the intrinsic chemical nature of nuclei and the precise localization of folate (FA) and luteiniz

SUBMITTER: Chen Y 

PROVIDER: S-EPMC4726017 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature