Stable multilineage xenogeneic replacement of definitive hematopoiesis in adult zebrafish.

ABSTRACT: Bony fishes are the most numerous and phenotypically diverse group of vertebrates inhabiting our planet, making them an ideal target for identifying general principles of tissue development and function. However, lack of suitable experimental platforms prevents the exploitation of this rich source of natural phenotypic variation. Here, we use a zebrafish strain lacking definitive hematopoiesis for interspecific analysis of hematopoietic cell development. Without conditioning prior to transplantation, hematopoietic progenitor cells from goldfish stably engraft in adult zebrafish homozygous for the c-myb(I181N) mutation. However, in competitive repopulation experiments, zebrafish hematopoietic cells exhibit an advantage over their goldfish counterparts, possibly owing to subtle species-speci

SUBMITTER: Hess I 

PROVIDER: S-EPMC4726038 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature