Genomic-assisted haplotype analysis and the development of high-throughput SNP markers for salinity tolerance in soybean.

ABSTRACT: Soil salinity is a limiting factor of crop yield. The soybean is sensitive to soil salinity, and a dominant gene, Glyma03g32900 is primarily responsible for salt-tolerance. The identification of high throughput and robust markers as well as the deployment of salt-tolerant cultivars are effective approaches to minimize yield loss under saline conditions. We utilized high quality (15x) whole-genome resequencing (WGRS) on 106 diverse soybean lines and identified three major structural variants and allelic variation in the promoter and genic regions of the GmCHX1 gene. The discovery of single nucleotide polymorphisms (SNPs) associated with structural variants facilitated the design of six KASPar assays. Additionally, haplotype analysis and pedigree tracking of 93 U.S. ancestral lines were perf

SUBMITTER: Patil G 

PROVIDER: S-EPMC4726057 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature