Topography induces differential sensitivity on cancer cell proliferation via Rho-ROCK-Myosin contractility.

ABSTRACT: Although the role of stiffness on proliferative response of cancer cells has been well studied, little is known about the effect of topographic cues in guiding cancer cell proliferation. Here, we examined the effect of topographic cues on cancer cell proliferation using micron scale topographic features and observed that anisotropic features like microgratings at specific dimension could reduce proliferation of non-cancer breast epithelial cells (MCF-10A) but not that for malignant breast cancer cells (MDA-MB-231 and MCF-7). However, isotropic features such as micropillars did not affect proliferation of MCF-10A, indicating that the anisotropic environmental cues are essential for this process. Interestingly, acto-myosin contraction inhibitory drugs, Y-27632 and blebbistatin prevented micr

SUBMITTER: Chaudhuri PK 

PROVIDER: S-EPMC4726280 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature