Biophysical regulation of Chlamydia pneumoniae-infected monocyte recruitment to atherosclerotic foci.

ABSTRACT: Chlamydia pneumoniae infection is implicated in atherosclerosis although the contributory mechanisms are poorly understood. We hypothesize that C. pneumoniae infection favors the recruitment of monocytes to atherosclerotic foci by altering monocyte biophysics. Primary, fresh human monocytes were infected with C. pneumoniae for 8 h, and the interactions between monocytes and E-selectin or aortic endothelium under flow were characterized by video microscopy and image analysis. The distribution of membrane lipid rafts and adhesion receptors were analyzed by imaging flow cytometry. Infected cells rolled on E-selectin and endothelial surfaces, and this rolling was slower, steady and uniform compared to uninfected cells. Infection decreases cholesterol levels, increases membrane fluidity, disrup

SUBMITTER: Evani SJ 

PROVIDER: S-EPMC4726309 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature