Transcriptomic Determinants of Scrapie Prion Propagation in Cultured Ovine Microglia.

ABSTRACT: Susceptibility to infection by prions is highly dependent on the amino acid sequence and host expression of the cellular prion protein (PrPC); however, cellular expression of a genetically susceptible PrPC is insufficient. As an example, it has been shown in cultured cells that permissive and resistant sublines derived from the same parental population often have similar expression levels of PrPC. Thus, additional cellular factors must influence susceptibility to prion infection. The aim of this study was to elucidate the factors associated with relative permissiveness and resistance to scrapie prions in cultured cells derived from a naturally affected species. Two closely related ovine microglia clones with different prion susceptibility, but no detectable differences in PrPC expression l

SUBMITTER: Munoz-Gutierrez JF 

PROVIDER: S-EPMC4726464 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature