Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry: results from an interim study.

ABSTRACT: There is an increasing interest for monitoring circulating myeloid-derived suppressor cells (MDSCs) in cancer patients, but there are also divergences in their phenotypic definition. To overcome this obstacle, the Cancer Immunoguiding Program under the umbrella of the Association of Cancer Immunotherapy is coordinating a proficiency panel program that aims at harmonizing MDSC phenotyping. After a consultation period, a two-stage approach was designed to harmonize MDSC phenotype. In the first step, an international consortium of 23 laboratories immunophenotyped 10 putative MDSC subsets on pretested, peripheral blood mononuclear cells of healthy donors to assess the level of concordance and define robust marker combinations for the identification of circulating MDSCs. At this stage, no manda

SUBMITTER: Mandruzzato S 

PROVIDER: S-EPMC4726716 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature