Response of porcine hepatocytes in primary culture to plasma from severe viral hepatitis patients.

ABSTRACT:

Aim

To observe the effects of plasma from patients with severe viral hepatitis (SVHP) on the growth and metabolism of porcine hepatocytes and the clinical efficiency of bioartificial liver device.

Methods

Hepatocytes were isolated from male porcines by collagenase perfusion. The synthesis of DNA and total protein, leakages of AST and LDH, changes in glutathione (GSH), catalase and morphology of porcine hepatocytes exposed to SVHP were investigated to indicate the effect of plasma from patients with severe hepatitis on the growth, injury, detoxification, and morphology of porcine hepatocytes.

Results

The synthesis of DNA and protein was inhibited in the medium containing 100% SVHP compared to the controls. The leakages of LDH and AST increased in porcine hepatocytes fo

SUBMITTER: Cheng YB 

PROVIDER: S-EPMC4727237 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature