Tbx5 is required for avian and Mammalian epicardial formation and coronary vasculogenesis.

ABSTRACT:

Rationale

Holt-Oram syndrome is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene. Overexpression of Tbx5 in the chick proepicardial organ impaired coronary blood vessel formation. However, the potential activity of Tbx5 in the epicardium itself, and the role of Tbx5 in mammalian coronary vasculogenesis, remains largely unknown.

Objective

To evaluate the consequences of altered Tbx5 gene dosage during proepicardial organ and epicardial development in the embryonic chick and mouse.

Methods and results

Retroviral-mediated knockdown or upregulation of Tbx5 expression in the embryonic chick proepicardial organ and proepicardial-specific deletion of Tbx5 in the embryonic mouse (Tbx5(epi-/)) impaired normal proepicardial organ cell development,

SUBMITTER: Diman NY 

PROVIDER: S-EPMC4727243 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature