Interferon regulatory factor 7 regulates airway epithelial cell responses to human rhinovirus infection.

ABSTRACT:

Background

Human rhinoviruses (HRV) cause the majority of colds and trigger exacerbations of chronic lower airway diseases. Airway epithelial cells are the primary site for HRV infection and replication, and the initiation of host inflammatory responses. At present, the molecular mechanisms that underpin HRV responses in airway epithelial cells are incompletely understood. The aim of this study was to employ microarray profiling, upstream regulator analysis, and siRNA mediated gene silencing to further our understanding of the role of interferon regulatory factor 7 (IRF7) in this response.

Methods

Primary human bronchial epithelial cells (HBE) where transfected with siRNA that targets IRF7 or a non-silencing control (all-star control) using Lipofectamine. The cells were allo

SUBMITTER: Bosco A 

PROVIDER: S-EPMC4727386 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature