Gene methylation profile of gastric cancerous tissue according to tumor site in the stomach.

ABSTRACT:

Background

There is considerable information on the methylation of the promoter regions of different genes involved in gastric carcinogenesis. However, there is a lack of information on how this epigenetic process differs in tumors originating at different sites in the stomach. The aim of this study is to assess the methylation profiles of the MLH1, MGMT, and DAPK-1 genes in cancerous tissues from different stomach sites.

Methods

Samples were acquired from 81 patients suffering stomach adenocarcinoma who underwent surgery for gastric cancer in the Lithuanian University of Health Sciences Hospital Kaunas Clinics in 2009-2012. Gene methylation was investigated with methylation-specific PCR. The study was approved by the Lithuanian Biomedical Research Ethics Committee.

Resul

SUBMITTER: Kupcinskaite-Noreikiene R 

PROVIDER: S-EPMC4727411 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature