Novel terpenes generated by heterologous expression of bacterial terpene synthase genes in an engineered Streptomyces host.

ABSTRACT: Mining of bacterial genome data has revealed numerous presumptive terpene synthases. Heterologous expression of several putative terpene synthase genes in an engineered Streptomyces host has revealed 13 newly discovered terpenes whose GC-MS and NMR data did not match with any known compounds in spectroscopic databases. Each of the genes encoding the corresponding terpene synthases were silent in their parent microorganisms. Heterologous expression and detailed NMR spectroscopic analysis allowed assignment of the structures of 13 new cyclic terpenes. Among these newly identified compounds, two were found to be linear triquinane sesquiterpenes that have never previously been isolated from bacteria or any other source. The remaining 11 new compounds were shown to be diterpene hydrocarbons and

SUBMITTER: Yamada Y 

PROVIDER: S-EPMC4727541 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature