The Hippo pathway mediates inhibition of vascular smooth muscle cell proliferation by cAMP.

ABSTRACT:

Aims

Inhibition of vascular smooth muscle cell (VSMC) proliferation by intracellular cAMP prevents excessive neointima formation and hence angioplasty restenosis and vein-graft failure. These protective effects are mediated via actin-cytoskeleton remodelling and subsequent regulation of gene expression by mechanisms that are incompletely understood. Here we investigated the role of components of the growth-regulatory Hippo pathway, specifically the transcription factor TEAD and its co-factors YAP and TAZ in VSMC.

Methods and results

Elevation of cAMP using forskolin, dibutyryl-cAMP or the physiological agonists, Cicaprost or adenosine, significantly increased phosphorylation and nuclear export YAP and TAZ and inhibited TEAD-luciferase report gene activity. Similar effects we

SUBMITTER: Kimura TE 

PROVIDER: S-EPMC4727789 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature