High Throughput Label Free Measurement of Cancer Cell Adhesion Kinetics Under Hemodynamic Flow.

ABSTRACT: The kinetics of receptor-mediated cell adhesion to extracellular matrix and adherent cell monolayers plays a key role in many physiological and pathological processes including cancer metastasis. Within this process the presence of fluidic shear forces is a key regulator of binding equilibrium and kinetics of cell adhesion. Current techniques to examine the kinetics of cell adhesion are either performed in the absence of flow or are low throughput, limiting their application to pharmacological compound screening or the high throughput investigation of biological mechanisms. We developed a high throughput flow device that applies flow in a multi-well format and interfaced this system with electric cell-substrate impedance sensing (ECIS) system to allow label free detection of cell adhesion.

SUBMITTER: Spencer A 

PROVIDER: S-EPMC4728493 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature