A Cell-Penetrating Peptide with a Guanidinylethyl Amine Structure Directed to Gene Delivery.

ABSTRACT: A peptide composed of lysine with a guanidinylethyl (GEt) amine structure in the side chain [Lys(GEt)] was developed as a cell-penetrating peptide directed to plasmid DNA (pDNA) delivery. The GEt amine adopted a diprotonated form at neutral pH, which may have led to the more efficient cellular uptake of a Lys(GEt)-peptide than an arginine-peptide at a low concentration. Lys(GEt)-peptide/pDNA complexes showed the highest transfection efficiency due to efficient endosomal escape without any cytotoxicity. Lys(GEt)-peptide may be a promising candidate as a gene delivery carrier.

SUBMITTER: Oba M 

PROVIDER: S-EPMC4728608 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature