Macrophage migration inhibitory factor enhances lipopolysaccharide-induced fibroblast proliferation by inducing toll-like receptor 4.

ABSTRACT:

Background

Fibroblast proliferation is a common manifestation of chronic inflammatory diseases, including rheumatoid arthritis (RA), Crohn's disease and ulcerative colitis, etc. To alleviate patient suffering, the mechanism underlying fibroblast proliferation should be elucidated.

Methods

CCK-8 assay was used to assess the stimulatory effect of LPS and macrophage migration inhibitory factor (MIF) on fibroblast proliferation. Then, TLR4 expression on fibroblast cell membrane was carried out by confocal scanning microscopy. Finally, real-time fluorescent quantitative PCR and flow cytometry were applied to determine the expression of TLR4 after MIF challenge.

Results

LPS alone directly stimulated the fibroblast proliferation. In addition, MIF showed co-stimulatory effect

SUBMITTER: Xi ZD 

PROVIDER: S-EPMC4728926 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature