Characterization of a novel metastatic prostate cancer cell line of LNCaP origin.

ABSTRACT:

Background

The LNCaP cell line was originally isolated from the lymph node of a patient with metastatic prostate cancer. Many cell lines have been derived from LNCaP by selective pressures to study different aspects of prostate cancer progression. When injected subcutaneously into male athymic nude mice, LNCaP and its derivatives rarely metastasize.

Methods

Here, we describe the characteristics of a new LNCaP derivative, JHU-LNCaP-SM, which was generated by long term passage in normal cell culture conditions.

Results

Short tandem repeat (STR) analysis and genomic sequencing verified JHU-LNCaP-SM derivation from parental LNCaP cells. JHU-LNCaP-SM cells express the same mutated androgen receptor (AR) but unlike LNCaP, are no longer androgen dependent for growth. The cel

SUBMITTER: Castanares MA 

PROVIDER: S-EPMC4729204 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature