Exploring Glycan Markers for Immunotyping and Precision-targeting of Breast Circulating Tumor Cells.

ABSTRACT:

Background and aims

Recognition of abnormal glycosylation in virtually every cancer type has raised great interest in exploration of the tumor glycome for biomarker discovery. Identifying glycan markers of circulating tumor cells (CTCs) represents a new development in tumor biomarker discovery. The aim of this study was to establish an experimental approach to enable rapid screening of CTCs for glycan marker identification and characterization.

Methods

We applied carbohydrate microarrays and a high-speed fiber-optic array scanning technology (FAST scan) to explore potential glycan markers of breast CTCs (bCTCs) and targeting antibodies. An anti-tumor monoclonal antibody, HAE3-C1 (C1), was identified as a key immunological probe in this study.

Results

In our carbohydra

SUBMITTER: Wang D 

PROVIDER: S-EPMC4729604 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature