Gene-expression analysis of cementoblasts and osteoblasts.

ABSTRACT:

Background and objective

Cementum and bone are similar mineralized tissues, but cementum accumulates much more slowly than bone, does not have vasculature or innervation and does not undergo remodeling. Despite these differences, there are no well-established markers to distinguish cementoblasts from other mature mineralizing cells such as osteoblasts and odontoblasts. The purpose of this study was to assess differences in gene expression between cementoblasts and osteoblasts using gene profiling of cell populations isolated directly from osteocalcin-green fluorescent protein (OC-GFP) transgenic mice.

Material and methods

OC-GFP reporter mice were used as they show labeling of cementoblasts, osteoblasts and odontoblasts, but not of periodontal ligament fibroblasts, within th

SUBMITTER: Matthews BG 

PROVIDER: S-EPMC4729669 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature