A shear gradient-activated microfluidic device for automated monitoring of whole blood haemostasis and platelet function.

ABSTRACT: Accurate assessment of blood haemostasis is essential for the management of patients who use extracorporeal devices, receive anticoagulation therapy or experience coagulopathies. However, current monitoring devices do not measure effects of haemodynamic forces that contribute significantly to platelet function and thrombus formation. Here we describe a microfluidic device that mimics a network of stenosed arteriolar vessels, permitting evaluation of blood clotting within small sample volumes under pathophysiological flow. By applying a clotting time analysis based on a phenomenological mathematical model of thrombus formation, coagulation and platelet function can be accurately measured in vitro in patient blood samples. When the device is integrated into an extracorporeal circuit in pig e

SUBMITTER: Jain A 

PROVIDER: S-EPMC4729824 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature