Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping.

ABSTRACT: Single-molecule techniques are being developed with the exciting prospect of revolutionizing the healthcare industry by generating vast amounts of genetic and proteomic data. One exceptionally promising route is in the use of nanopore sensors. However, a well-known complexity is that detection and capture is predominantly diffusion limited. This problem is compounded when taking into account the capture volume of a nanopore, typically 10(8)-10(10) times smaller than the sample volume. To rectify this disproportionate ratio, we demonstrate a simple, yet powerful, method based on coupling single-molecule dielectrophoretic trapping to nanopore sensing. We show that DNA can be captured from a controllable, but typically much larger, volume and concentrated at the tip of a metallic nanopore. Th

SUBMITTER: Freedman KJ 

PROVIDER: S-EPMC4729827 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature