A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization.

ABSTRACT: Intramolecular photostabilization via triple-state quenching was recently revived as a tool to impart synthetic organic fluorophores with 'self-healing' properties. To date, utilization of such fluorophore derivatives is rare due to their elaborate multi-step synthesis. Here we present a general strategy to covalently link a synthetic organic fluorophore simultaneously to a photostabilizer and biomolecular target via unnatural amino acids. The modular approach uses commercially available starting materials and simple chemical transformations. The resulting photostabilizer-dye conjugates are based on rhodamines, carbopyronines and cyanines with excellent photophysical properties, that is, high photostability and minimal signal fluctuations. Their versatile use is demonstrated by single-step

SUBMITTER: van der Velde JH 

PROVIDER: S-EPMC4729898 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature