Seed-Specific Expression of Spider Silk Protein Multimers Causes Long-Term Stability.

ABSTRACT: Seeds enable plants to germinate and to grow in situations of limited availability of nutrients. The stable storage of different seed proteins is a remarkable presumption for successful germination and growth. These strategies have been adapted and used in several molecular farming projects. In this study, we explore the benefits of seed-based expression to produce the high molecular weight spider silk protein FLAG using intein-based trans-splicing. Multimers larger than 460 kDa in size are routinely produced, which is above the native size of the FLAG protein. The storage of seeds for 8 weeks and 1 year at an ambient temperature of 15°C does not influence the accumulation level. Even the extended storage time does not influence the typical pattern of multimerized bands. These results show

SUBMITTER: Weichert N 

PROVIDER: S-EPMC4729946 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature