Column Selection for Biomedical Analysis Supported by Column Classification Based on Four Test Parameters.

ABSTRACT: This article focuses on correlating the column classification obtained from the method created at the Katholieke Universiteit Leuven (KUL), with the chromatographic resolution attained in biomedical separation. In the KUL system, each column is described with four parameters, which enables estimation of the FKUL value characterising similarity of those parameters to the selected reference stationary phase. Thus, a ranking list based on the FKUL value can be calculated for the chosen reference column, then correlated with the results of the column performance test. In this study, the column performance test was based on analysis of moclobemide and its two metabolites in human plasma by liquid chromatography (LC), using 18 columns. The comparative study was performed using traditional correl

SUBMITTER: Plenis A 

PROVIDER: S-EPMC4730375 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature