Cloning and purification of the first termicin-like peptide from the cockroach Eupolyphaga sinensis.

ABSTRACT:

Background

Termicin is an antimicrobial peptide with six cysteines forming three disulfide bridges that was firstly isolated from the salivary glands and hemocytes of the termite Pseudacanthotermes spiniger. In contrast to many broad-spectrum antimicrobial peptides, termicin is most active against filamentous fungi. Although more than one hundred complementary DNAs (cDNAs) encoding termicin-like peptides have been reported to date, all these termicin-like peptides were obtained from Isoptera insects.

Methods

The cDNA was cloned by combination of cDNA library construction kit and DNA sequencing. The polypeptide was purified by gel filtration and reversed-phase high performance liquid chromatography (RP-HPLC). Its amino acid sequence was determined by Edman degradation and mas

SUBMITTER: Liu Z 

PROVIDER: S-EPMC4730610 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature