De novo construction of an expanded transcriptome assembly for the western tarnished plant bug, Lygus hesperus.

ABSTRACT:

Background

The plant bug Lygus hesperus Knight is a polyphagous pest of many economically important crops. Despite its pest status, little is known about the molecular mechanisms responsible for much of the biology of this species. Earlier Lygus transcriptome assemblies were limited by low read depth, or because they focused on specific conditions. To generate a more comprehensive transcriptome, we supplemented previous datasets with new reads corresponding to specific tissues (heads, antennae, and male reproductive tissues). This transcriptome augments current Lygus molecular resources and provides the foundational knowledge critical for future comparative studies.

Findings

An expanded, Trinity-based de novo transcriptome assembly for L. hesperus was generated using previou

SUBMITTER: Tassone EE 

PROVIDER: S-EPMC4730634 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature