Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer.

ABSTRACT:

Background

The miniaturised and portable DNA sequencer MinION™ has been released to the scientific community within the framework of an early access programme to evaluate its application for a wide variety of genetic approaches. This technology has demonstrated great potential, especially in genome-wide analyses. In this study, we tested the ability of the MinION™ system to perform amplicon sequencing in order to design new approaches to study microbial diversity using nearly full-length 16S rDNA sequences.

Results

Using R7.3 chemistry, we generated more than 3.8 million events (nt) during a single sequencing run. These data were sufficient to reconstruct more than 90 % of the 16S rRNA gene sequences for 20 different species present in a mock reference community. After read

SUBMITTER: Benitez-Paez A 

PROVIDER: S-EPMC4730766 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature