Impact of Freezing Delay Time on Tissue Samples for Metabolomic Studies.

ABSTRACT:

Introduction

Metabolic profiling of intact tumor tissue by high-resolution magic angle spinning (HR MAS) MR spectroscopy (MRS) provides important biological information possibly useful for clinical diagnosis and development of novel treatment strategies. However, generation of high-quality data requires that sample handling from surgical resection until analysis is performed using systematically validated procedures. In this study, we investigated the effect of postsurgical freezing delay time on global metabolic profiles and stability of individual metabolites in intact tumor tissue.

Materials and methods

Tumor tissue samples collected from two patient-derived breast cancer xenograft models (n = 3 for each model) were divided into pieces that were snap-frozen in liquid nitr

SUBMITTER: Haukaas TH 

PROVIDER: S-EPMC4730796 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature