Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.

ABSTRACT:

Background & aims

Intestinal microfold (M) cells are specialized epithelial cells that act as gatekeepers of luminal antigens in the intestinal tract. They play a critical role in the intestinal mucosal immune response through transport of viruses, bacteria and other particles and antigens across the epithelium to immune cells within Peyer's patch regions and other mucosal sites. Recent studies in mice have demonstrated that M cells are generated from Lgr5+ intestinal stem cells (ISCs), and that infection with Salmonella enterica serovar Typhimurium increases M cell formation. However, it is not known whether and how these findings apply to primary human small intestinal epithelium propagated in an in vitro setting.

Methods

Human intestinal crypts were grown as monolayers wi

SUBMITTER: Rouch JD 

PROVIDER: S-EPMC4731053 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature