A Unidirectional Cell Switching Gate by Engineering Grating Length and Bending Angle.

ABSTRACT: On a microgrooved substrate, cells migrate along the pattern, and at random positions, reverse their directions. Here, we demonstrate that these reversals can be controlled by introducing discontinuities to the pattern. On "V-shaped grating patterns", mouse osteogenic progenitor MC3T3-E1 cells reversed predominately at the bends and the ends. The patterns were engineered in a way that the combined effects of angle- and length-dependence could be examined in addition to their individual effects. Results show that when the bend was placed closer to one end, migration behaviour of cells depends on their direction of approach. At an obtuse bend (135°), more cells reversed when approaching from the long segment than from the short segment. But at an acute bend (45°), this relationship was rever

SUBMITTER: Zhou SF 

PROVIDER: S-EPMC4731054 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature