In vitro cytotoxic and in silico activity of piperine isolated from Piper nigrum fruits Linn.

ABSTRACT:

Background

Piper nigrum [Piperaceae], commonly known as black pepper is used as medicine fairly throughout the greater part of India and as a spice globally.

Purpose

To isolate piperine and evaluate in vitro cytotoxic [antiproliferative] activity and in silico method.

Methods

Piperine was isolated from the fruits of P.nigrum. Piperine was characterized by UV,IR, (1)H-NMR, (13)C-NMR and Mass spectrum. Standardization of piperine was done also by HPTLC fingerprinting. In vitro cytotoxic activity was done using HeLa cell lines by MTT assay at different concentrations ranging from 20 to 100 μg/ml in triplicate and in silico docking studies using enzyme EGFR tyrosine kinase.

Results

Fingerprinting of isolated piperine were done by HPTLC method. The IC50 value was fo

SUBMITTER: Paarakh PM 

PROVIDER: S-EPMC4731375 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature