Characterization of arginine kinase, anovel allergen of dermatophagoides farinae (Der f 20).

ABSTRACT:

Objective

To characterize a novel allergen, the Dermatophagoides farinae-derived arginine kinase (Der f 20).

Methods

The protein of Der f 20 was synthesized by genetic engineering approaches. The allergenicity of Der f 20 was tested by enzyme-linked immunosorbent assay and an airway allergy mouse model.

Results

The Der f 20 gene was cloned andpresented in the Gene Bank with an accession number of AAP57094. The Der f 20 is an arginine kinase (AK), whichshowed a close relationship with D. pteronyssinus AK and Aleuroglyphusovatus AK. Western-blot and ELISA studies showed the IgE binding capacity of Der f 20 was 66.7% in the sera from 6 dust mite allergic patients. Immune inhibition assayresults showed the IgE cross-reactivity between Der f 20 and DME (Dust mite extract).

SUBMITTER: Xing P 

PROVIDER: S-EPMC4731678 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature