A human beta cell line with drug inducible excision of immortalizing transgenes.

ABSTRACT:

Objectives

Access to immortalized human pancreatic beta cell lines that are phenotypically close to genuine adult beta cells, represent a major tool to better understand human beta cell physiology and develop new therapeutics for Diabetes. Here we derived a new conditionally immortalized human beta cell line, EndoC-βH3 in which immortalizing transgene can be efficiently removed by simple addition of tamoxifen.

Methods

We used lentiviral mediated gene transfer to stably integrate a tamoxifen inducible form of CRE (CRE-ERT2) into the recently developed conditionally immortalized EndoC βH2 line. The resulting EndoC-βH3 line was characterized before and after tamoxifen treatment for cell proliferation, insulin content and insulin secretion.

Results

We showed that EndoC-βH

SUBMITTER: Benazra M 

PROVIDER: S-EPMC4731729 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature