Hepatocyte TRAF3 promotes insulin resistance and type 2 diabetes in mice with obesity.

ABSTRACT:

Objective

Metabolic inflammation is believed to promote insulin resistance and type 2 diabetes progression in obesity. TRAF3, a cytoplasmic signaling protein, has been known to mediate/modulate cytokine signaling in immune cells. The goal is to define the metabolic function of hepatic TRAF3 in the setting of obesity.

Methods

Hepatocyte-specific TRAF3 knockout mice were generated using the loxp/albumin-cre system. Liver TRAF3 was deleted in adult obese mice via Cre adenoviral infection. Both high fat diet-induced and genetic obesity were examined. TRAF3 levels and insulin signaling were measured by immunoblotting. Insulin sensitivity, hepatic glucose production, and glucose metabolism were examined by glucose, insulin, and pyruvate tolerance tests. Hepatic steatosis was exami

SUBMITTER: Chen Z 

PROVIDER: S-EPMC4731737 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature