Fast and precise targeting of single tumor cells in vivo by multimodal correlative microscopy.

ABSTRACT: Intravital microscopy provides dynamic understanding of multiple cell biological processes, but its limited resolution has so far precluded structural analysis. Because it is difficult to capture rare and transient events, only a few attempts have been made to observe specific developmental and pathological processes in animal models using electron microscopy. The multimodal correlative approach that we propose here combines intravital microscopy, microscopic X-ray computed tomography and three-dimensional electron microscopy. It enables a rapid (c.a. 2 weeks) and accurate (<5 µm) correlation of functional imaging to ultrastructural analysis of single cells in a relevant context. We demonstrate the power of our approach by capturing single tumor cells in the vasculature of the cerebral cor

SUBMITTER: Karreman MA 

PROVIDER: S-EPMC4732291 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature