Shotgun proteomics as a viable approach for biological discovery in the Pacific oyster.

ABSTRACT: Shotgun proteomics offers an efficient means to characterize proteins in a complex mixture, particularly when sufficient genomic resources are available. In order to assess the practical application of shotgun proteomics in the Pacific oyster (Crassostrea gigas), liquid chromatography coupled with tandem mass spectrometry was used to characterize the gill proteome. Using information from the recently published Pacific oyster genome, 1043 proteins were identified. Biological samples (n = 4) and corresponding technical replicates (three) were similar in both specific proteins identified and expression, as determined by normalized spectral abundance factor. A majority of the proteins identified (703) were present in all biological samples. Functional analysis of the protein repertoire illustr

SUBMITTER: Timmins-Schiffman E 

PROVIDER: S-EPMC4732435 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature