Serological, genomic and structural analyses of the major mite allergen Der p 23.

ABSTRACT:

Background

Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein.

Objective

This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population and to determine the structure for functional characterization.

Methods

IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7 and Der p 8 was measured by ELISA. RNA-seq data from D. pteronyssinus were compared as estimates of allergen expression levels. The structure was analysed by X-ray crystallography and NMR.

Results

Despite a high prevalence of Der p 23, (75% vs. 87% and 79% for Der p 1 and Der p 2, respectively), the anti-Der p 23 IgE levels were relatively low. The patient response to th

SUBMITTER: Mueller GA 

PROVIDER: S-EPMC4732897 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature