Ionizing Radiation Impacts on Cardiac Differentiation of Mouse Embryonic Stem Cells.

ABSTRACT: Little is known about the effects of ionizing radiation on the earliest stages of embryonic development although it is well recognized that ionizing radiation is a natural part of our environment and further exposure may occur due to medical applications. The current study addresses this issue using D3 mouse embryonic stem cells as a model system. Cells were irradiated with either X-rays or carbon ions representing sparsely and densely ionizing radiation and their effect on the differentiation of D3 cells into spontaneously contracting cardiomyocytes through embryoid body (EB) formation was measured. This study is the first to demonstrate that ionizing radiation impairs the formation of beating cardiomyocytes with carbon ions being more detrimental than X-rays. However, after prolonged cul

SUBMITTER: Helm A 

PROVIDER: S-EPMC4733326 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature