Scleral fibroblast response to experimental glaucoma in mice.

ABSTRACT:

Purpose

To study the detailed cellular and molecular changes in the mouse sclera subjected to experimental glaucoma.

Methods

Three strains of mice underwent experimental bead-injection glaucoma and were euthanized at 3 days and 1, 3, and 6 weeks. Scleral protein expression was analyzed with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using (16)O/(18)O labeling for quantification in 1- and 6-week tissues. Sclera protein samples were also analyzed with immunoblotting with specific antibodies to selected proteins. The proportion of proliferating scleral fibroblasts was quantified with Ki67 and 4',6-diamidino-2-phenylindole (DAPI) labeling, and selected proteins were studied with immunohistochemistry.

Results

Proteomic analysis showed increases

SUBMITTER: Oglesby EN 

PROVIDER: S-EPMC4734151 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature