Toward Contactless Biology: Acoustophoretic DNA Transfection.

ABSTRACT: Acoustophoresis revolutionized the field of container-less manipulation of liquids and solids by enabling mixing procedures which avoid contamination and loss of reagents due to the contact with the support. While its applications to chemistry and engineering are straightforward, additional developments are needed to obtain reliable biological protocols in a contactless environment. Here, we provide a first, fundamental step towards biological reactions in air by demonstrating the acoustophoretic DNA transfection of mammalian cells. We developed an original acoustophoretic design capable of levitating, moving and mixing biological suspensions of living mammalians cells and of DNA plasmids. The precise and sequential delivery of the mixed solutions into tissue culture plates is actuated by

SUBMITTER: Vasileiou T 

PROVIDER: S-EPMC4734324 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature