Methylation Markers for the Identification of Body Fluids and Tissues from Forensic Trace Evidence.

ABSTRACT: The identification of body fluids is an essential tool for clarifying the course of events at a criminal site. The analytical problem is the fact that the biological material has been very often exposed to detrimental exogenous influences. Thereby, the molecular substrates used for the identification of the traces may become degraded. So far, most protocols utilize cell specific proteins or RNAs. Instead of measuring these more sensitive compounds this paper describes the application of the differential DNA-methylation. As a result of two genome wide screenings with the Illumina HumanMethylation BeadChips 27 and 450k we identified 150 candidate loci revealing differential methylation with regard to the body fluids venous blood, menstrual blood, vaginal fluid, saliva and sperm. Among them w

SUBMITTER: Forat S 

PROVIDER: S-EPMC4734623 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature