De Novo Evolutionary Emergence of a Symmetrical Protein Is Shaped by Folding Constraints.

ABSTRACT: Molecular evolution has focused on the divergence of molecular functions, yet we know little about how structurally distinct protein folds emerge de novo. We characterized the evolutionary trajectories and selection forces underlying emergence of β-propeller proteins, a globular and symmetric fold group with diverse functions. The identification of short propeller-like motifs (<50 amino acids) in natural genomes indicated that they expanded via tandem duplications to form extant propellers. We phylogenetically reconstructed 47-residue ancestral motifs that form five-bladed lectin propellers via oligomeric assembly. We demonstrate a functional trajectory of tandem duplications of these motifs leading to monomeric lectins. Foldability, i.e., higher efficiency of folding, was the main paramet

SUBMITTER: Smock RG 

PROVIDER: S-EPMC4735018 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature