Lectin binding of human sperm associates with DEFB126 mutation and serves as a potential biomarker for subfertility.

ABSTRACT: Coating on the sperm surface, glycocalyx, plays a key role in sperm motility, maturation and fertilization. A comprehensive profile of sperm surface glycans will greatly facilitate both basic researches and clinical studies. Because of the capability of recognizing different glycan moieties, lectins are widely used in glycobiology. However, lacking high-throughput technology, limited lectins have been reported for analyzing the glycan of human sperm. In this study, we employed a lectin microarray for profiling the surface glycans of human sperm, on which 54 out of 91 lectins showed positive binding. Based on this technique, we compared lectin binding profiling of sperm with homozygous DEFB126 mutation (del/del) with that of wild type (wt/wt). DEFB126 was reported to contribute to the sialy

SUBMITTER: Xin A 

PROVIDER: S-EPMC4735291 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature