Combined local and systemic immunization is essential for durable T-cell mediated heterosubtypic immunity against influenza A virus.

ABSTRACT: The threat from unpredictable influenza virus pandemics necessitates the development of a new type of influenza vaccine. Since the internal proteins are highly conserved, induction of T cells targeting these antigens may provide the solution. Indeed, adenoviral (Ad) vectors expressing flu nucleoprotein have previously been found to induce short-term protection in mice. In this study we confirm that systemic (subcutaneous (s.c.) immunization rapidly induced heterosubtypic protection predominantly mediated by CD8 T cells, but within three months clinical protection completely disappeared. Local (intranasal (i.n.)) immunization elicited delayed, but more lasting protection despite relatively inefficient immunization. However, by far, the most robust protection was induced by simultaneous, com

SUBMITTER: Uddback IE 

PROVIDER: S-EPMC4735591 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature